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Life Illuminated: Selected Papers from Cold Spring Harbor
Volume 2, 1972-1994

Subject Area(s):  Cold Spring Harbor Laboratory HistoryHistory of Science

Edited by Jan A. Witkowski, Cold Spring Harbor Laboratory, New York; Alexander Gann, Cold Spring Harbor Laboratory, New York; Joseph F. Sambrook, Peter MacCallum Cancer Institute, East Melbourne, Australia

© 2008 • 242 pp., illustrations from original articles, indexes, CD
Hardcover •
ISBN  978-087969804-1

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This book is the second volume of an intellectual history of the science done at CSHL (the first volume, Illuminating Life, showed that genetics became the dominant theme of research at CSH by as early as 1904). The appointment of James Watson as Director of the Laboratory in 1968 set off the explosive research development at CSH, as he recruited widely and wisely teams of investigators with diverse scientific interests. From this collection of papers, presented in full on the accompanying CD, several themes emerge: the characterization and exploitation of mobile genetic elements; the mechanics of DNA replication and regulation of the cell cycle; the behavior and internal architecture of cells; how viruses induce tumors; the discovery of cancer genes; the characteristics of neurons; and the invention of techniques that make possible further progress. Each theme is introduced in the context of the science of the time, and each paper has a commentary by, in most cases, one of its authors. Life Illuminated is a story of scientific innovation and achievement, told in the words of the investigators themselves.


Jan A. Witkowski, Alexander Gann, and Joseph F. Sambrook

Gerald R. Fink
Introduction: Genes Are Interesting to Geneticists
Rasika Harshey
Ljungquist E. and Bukhari A.I. 1977. State of prophage Mu DNA upon induction. Proc. Natl. Acad. Sci. 74: 3143-3147.
Bukhari Memoriam
Taylor A.L. and Szybalski W. 1984. In memoriam: Ahmad I. Bukhari, 1943-1983. Gene 27: iii-iv.
James B. Hicks
Strathern J.N, Klar A.J.S., Hicks J.B., Abraham J.A., Ivy J.M., Nasmyth K.A., and McGill C. 1982. Homothallic switching of yeast mating type cassettes is initiated by a double-stranded cut in the MAT locus. Cell 31: 183-192.
Robert Martienssen
Sundaresan V., Springer P., Volpe T., Haward S., Jones J.D.G., Dean C., Ma H., and Martienssen R. 1995. Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements. Genes Dev. 9: 1797-1810.
David Beach
Draetta G., Luca F.,Westendorf J., Brizuela L., Ruderman J., and Beach D. 1989. cdc2 protein kinase is complexed with both cyclin A and B: Evidence for proteolytic inactivation of MPF. Cell 56: 829-838.

Bruce Alberts
Introduction: Why the Revolution That Jim Started Continues
Joseph F. Sambrook
Watson J.D. 1972. Origin of concatemeric T7 DNA. Nat. New Biol. 239: 197-201.
Bruce Stillman
Prelich G., Kostura M., Marshak D.R., Mathews M.B., and Stillman B. 1987. The cell cycle regulated proliferating cell nuclear antigen is required for SV40 DNA replication in vitro. Nature 326: 471-475.
Bruce Stillman
Bell S.P. and Stillman B. 1992. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex. Nature 357: 128-134.
Richard J. Roberts
Klimašauskas S., Kumar S., Roberts R.J., and Cheng X. 1994. HhaI methyltransferase flips its target base out of the DNA helix. Cell 76: 357-369.

David L. Spector
Introduction: From Architecture to Functional Analysis
Klaus Weber
Lazarides E. and Weber K. 1974. Actin antibody: The specific visualization of actin filaments in non-muscle cells. Proc. Natl. Acad. Sci. 71: 2268-2272.
Guenter Albrecht-Buehler
Albrecht-Buehler G. 1977. Daughter 3T3 cells. Are they mirror images of each other? J. Cell Biol. 72: 595-603.
Dafna Bar-Sagi and James R. Feramisco
Bar-Sagi D. and Feramisco J.R. 1985. Microinjection of the ras oncogene protein into PC12 cells induces morphological differentiation. Cell 42: 841-848.
Carol W. Greider
Harley C.B., Futcher A.B., and Greider C.W. 1990. Telomeres shorten during ageing of human fibroblasts. Nature 345: 458-460.
David L. Spector
Spector D.L. 1990. Higher order nuclear organization: Three-dimensional distribution of small nuclear ribonucleoprotein particles. Proc. Natl. Acad. Sci. 87: 147-151.

Joseph F. Sambrook
Introduction: The Early Days of Tumor Virus Research at Cold Spring Harbor
Joseph F. Sambrook
Sambrook J., Sharp P.A., and Keller W. 1972. Transcription of simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J. Mol. Biol. 70: 57-71.
Terri Grodzicker
Grodzicker T., Williams J., Sharp P., and Sambrook J. 1974. Physical mapping of temperature-sensitive mutations of adenoviruses. Cold Spring Harbor Symp. Quant. Biol. 39(Pt 1): 439-446.
Louise T. Chow
Chow L.T., Gelinas R.E., Broker T.R., and Roberts R.J. 1977. An amazing sequence arrangement at the 5’ ends of adenovirus 2 messenger RNA. Cell 12: 1-8.
Michael Botchan
Botchan M., Topp W., and Sambrook J. 1976. The arrangement of simian virus 40 sequences in the DNA of transformed cells. Cell 9: 269-287.
Robert Tjian
Tjian R. 1978. The binding site on SV40 DNA for a T antigen-related protein. Cell 13: 165-179.
Joseph F. Sambrook
Gluzman Y. 1981. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182.
Carl S. Thummel
Thummel C., Tjian R., and Grodzicker T. 1981. Expression of SV40 T antigen under control of adenovirus promoters. Cell 23: 825-836.
Winship Herr
Herr W. and Clarke J. 1986. The SV40 enhancer is composed of multiple functional elements that can compensate for one another. Cell 45: 461-470.

Eric Kandel
Introduction: Neuroscience at CSH Laboratory: Meetings, Courses, and Research
Ron McKay
Introduction: Getting the Point at Cold Spring Harbor
Birgit Zipser
Zipser B. and McKay R. 1981. Monoclonal antibodies distinguish identifiable neurons in the leech. Nature 289: 549-554.
Ron McKay
Hockfield S. and McKay R.D.G. 1985. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5: 3310-3328.
Tim Tully
Yin J.C.P.,Wallach J.S., Del Vecchio M.,Wilder E.L., Zhou H., Quinn W.G., and Tully T. 1994. Induction of a dominant negative CREB transgene specifically blocks longterm memory in Drosophila. Cell 79: 49-58.

Arnold J. Levine
Introduction: The Road to Understanding the Origins of Cancer in Humans
Mitchell Goldfarb
Goldfarb M., Shimizu K., Perucho M., and Wigler M. 1982. Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells. Nature 296: 404-409.
Earl Ruley
Ruley H.E. 1983. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature 304: 602-606.
Scott Powers
Kataoka T., Powers S., Cameron S., Fasano O., Goldfarb M., Broach J., and Wigler M. 1985. Functional homology of mammalian and yeast RAS genes. Cell 40: 19-26.
Douglas Hanahan
Hanahan D. 1985. Heritable formation of pancreatic β-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenes. Nature 315: 115-122.
B. Robert Franza, Jr.
Franza B.R. Jr., Rauscher F.J. 3rd, Josephs S.F., and Curran T. 1988. The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites. Science 239: 1150-1153.
Ed Harlow
Whyte P., Buchkovich K.J., Horowitz J.M, Friend S.H., Raybuck M., Weinberg R.A., and Harlow E. 1988. Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product. Nature 334: 124-129.

Angela N.H. Creager
Introduction: Early Technological Breakthroughs at Cold Spring Harbor
Richard J. Roberts
Roberts R.J. 1978. Restriction and modification enzymes and their recognition sequences. Gene 4: 183-194.
James I. Garrels
Garrels J.I. 1989. The QUEST system for quantitative analysis of two-dimensional gels. J. Biol. Chem 264: 5269-5282.
Joseph F. Sambrook
Sharp P.A., Sugden B., and Sambrook J. 1973. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose-ethidium bromide electrophoresis. Biochemistry 12: 3055-3063.
Tom Maniatis
Maniatis T., Kee S.G., Efstratiadis A., and Kafatos F.C. 1976. Amplification and characterization of a β-globin gene synthesized in vitro. Cell 8: 163-182.

Author Index



“Perhaps most importantly, this is a volume about life at Cold Spring Harbor. Very few pages pass without a mention of some word or deed by James Watson and the presence of Barbara McClintock is felt throughout the book. There are fascinating descriptions of Watson making his nightly rounds of the research laboratories, and of him wryly reminding a postdoctoral researcher caught building a model of DNA: ‘[t]hat's been done’. Tim Tully tells an interesting tale of Watson’s willingness to support a long and often frustrating assault on the biology of memory.

In an early chapter, Bruce Alberts reminds us that ‘cells are much smarter than scientists’. True enough, but the denizens of CSHL in the 1970s and 1980s were (and still are) rapidly closing the gap.”

      —The Quarterly Review of Biology