© 2018 • 426 pages, illustrated (136 color and 64 B&W), index
Paperback • $120.00 • ISBN 978-1-621822-87-5

The 82nd Cold Spring Harbor Symposium focused on Chromosome Segregation & Structure and addressed the enormous progress in our understanding of the nature and behavior of chromosomes during the life cycle of the cell. It is rare to find such a wide-ranging perspective on this topic in one volume and this collection of papers will be valuable to investigators interested in many aspects of cell biology, genetics, and cancer. The topics covered at the meeting included Meiosis; Mitosis; Chromosome Segregation; Centrosomes and Centrioles; Ploidy, Chromosome Segregation Errors, and Disease; Asymmetric Cell Division; Nuclear Architecture; Chromosome Structure and Condensation; Sister Chromatid Cohesion; Genome Stability; and Germ Cells. Numerous speakers participated in interviews during the course of the Symposium week and transcripts of those discussions and the Dorcas Cummings lecture by David Page are included.

Symposium Participants

Foreword

Chromatin, Histones, And Instability



Shaping Chromatin in the Nucleus: The Bricks and the Architects

David Sitbon, Katrina Podsypanina, Tejas Yadav, and Geneviève Almouzni



The Importance of Satellite Sequence Repression for Genome Stability

Peter Zeller and Susan M. Gasser



Structure and Epigenetic Regulation of Chromatin Fibers

Ping Chen and Guohong Li



Chromosome Biology and Genome Architecture



The Role of Bromodomain and Extraterminal Motif (BET) Proteins in Chromatin Structure

Sarah C. Hsu and Gerd A. Blobel



Emerging Evidence of Chromosome Folding by Loop Extrusion

Geoffrey Fudenberg, Nezar Abdennur, Maxim Imakaev, Anton Goloborodko and Leonid A. Mirny



SpotLearn: Convolutional Neural Network for Detection of Fluorescence In Situ Hybridization (FISH) Signals in High-Throughput Imaging Approaches

Prabhakar R. Gudla, Koh Nakayama, Gianluca Pegoraro, and Tom Misteli



Centromeres and Kinetochores



Remarkable Evolutionary Plasticity of Centromeric Chromatin

Steven Henikoff, Jitendra Thakur, Sivakanthan Kasinathan, and Paul B. Talbert



Molecular Structures of Yeast Kinetochore Subcomplexes and Their Roles in Chromosome Segregation

Simon Jenni, Yoana N. Dimitrova, Roberto Valverde, Stephen M. Hinshaw, and Stephen C. Harrison



Aurora A Kinase Function at Kinetochores

Jennifer DeLuca



RotoStep: A Chromosome Dynamics Simulator Reveals Mechanisms of Loop Extrusion

Josh Lawrimore, Brandon Friedman, Ayush Doshi, and Kerry Bloom



Taming the Beast: Control of APC/CCdc20-Dependent Destruction

Pablo Lara-Gonzalez, Taekyung Kim, and Arshad Desai



Organizing Chromosomes during Segregation



Noncanonical Biogenesis of Centrioles and Basal Bodies

Catarina Nabais, Sónia Gomes Pereira, and Mónica Bettencourt-Dias



Impact of Centrosome Aberrations on Chromosome Segregation and Tissue Architecture in Cancer

Erich A. Nigg, Dominik Schnerch, and Olivier Ganier



The ABCs of Centriole Architecture: The Form and Function of Triplet Microtubules

Jennifer T. Wang and Tim Stearns



Mitotic Chromosome Assembly In Vitro: Functional Cross Talk between Nucleosomes and Condensins

Keishi Shintomi and Tatsuya Hirano



Mitosis and Cell Cycle



Spindle-to-Cortex Communication in Cleaving Frog Eggs

Timothy J. Mitchison and Christine M. Field



Nucleosome-Dependent Pathways That Control Mitotic Progression

Hironori Funabiki, Christopher Jenness, and Christian Zierhut



Knotty Problems during Mitosis: Mechanistic Insight into the Processing of Ultrafine DNA Bridges in Anaphase

Kata Sarlós, Andreas Biebricher, Erwin J.G. Petermann, Gijs J.L. Wuite, and Ian D. Hickson



Low-Level, Global Transcription during Mitosis and Dynamic Gene Reactivation during Mitotic Exit

Katherine C. Palozola, Hong Liu, Dario Nicetto, and Kenneth S. Zaret



Genome Instability as a Consequence of Defects in the Resolution of Recombination Intermediates

Stephen C. West and Ying Wai Chan



Meiosis



Reconstitution of Female Germ Cell Fate Determination and Meiotic Initiation in Mammals

So I. Nagaoka and Mitinori Saitou



Regulation of Crossover Frequency and Distribution during Meiotic Recombination

Takamune T. Saito and Monica P. Colaiácovo



Oocyte Quality Control: Causes, Mechanisms, and Consequences

Neil Hunter



Cellular and Molecular Mechanisms of Centromere Drive

Michael A. Lampson and Ben E. Black



Hierarchical Regulation of Centromeric Cohesion Protection by Meikin and Shugoshin during Meiosis I

Seira Miyazaki, Jihye Kim, Takeshi Sakuno, and Yoshinori Watanabe



Germ Cells, Imprinting, Gene Dosage, and Regulation



Topologically Associating Domains in Chromosome Architecture and Gene Regulatory Landscapes during Development, Disease and Evolution

Rafael Galupa and Edith Heard



Dynamic Control of Chromosome Topology and Gene Expression by a Chromatin Modification

Qian Bian, Erika C. Anderson, Katjuša Brejc, and Barbara J. Meyer



Polytene Chromosome Structure and Somatic Genome Instability

Allan C. Spradling



Heterochromatin, Errors, And Damage



Symmetry from Asymmetry or Asymmetry from Symmetry?

Elizabeth W. Kahney, Rajesh Ranjan, Ryan J. Gleason, and Xin Chen



Function of Junk: Pericentromeric Satellite DNA in Chromosome Maintenance

Madhav Jagannathan and Yukiko M. Yamashita



Homologous Recombination and Replication Fork Protection: BRCA2 and More!

Weiran Feng and Maria Jasin



Fork Protection and Therapy Resistance in Hereditary Breast Cancer

Sharon B. Cantor and Jennifer A. Calvo



Summary



How the Genome Folds, Divides, Lives, and Dies

Whitney L. Johnson, Kathleen T. Xie Mijung Kwon, Shiwei Liu, and David Pellman

Dorcas Cummings Lecture



David Page

Conversations at the Symposium



Marisa Bartolomei



Mónica Bettencourt-Dias



Kerry Bloom



Iain Cheeseman



Monica P. Colaiácovo



Bernard de Massy



Arshad Desai



Daniel Durocher



Steve Henikoff



Martin Hetzer



Ian Hickson



Maria Jasin



Leonid Mirny



Timothy Mitchison



Tim Stearns



Aaron Straight



Yukiko Yamashita



Author Index

Subject Index